Use of the Chrome Azurol S Agar Plate Technique To Differentiate Strains and Field Isolates of Rhizobium leguminosarum biovar trifolii.

Direct amplification of nodD from community DNA reveals the genetic diversity of Rhizobium leguminosarum in soil.

Sequences of nodD, a gene found only in rhizobia, were amplified from total community DNA isolated from a pasture soil. The polymerase chain reaction (PCR) primers used, Y5 and Y6, match nodD from Rhizobium leguminosarum biovar trifolii, R. leguminosarum biovar viciae and Sinorhizobium meliloti. The PCR product was cloned and yielded 68 clones that were identified by restriction pattern as deri...

Cell-bound Cellulase and Polygalacturonase Production by Rhizobium and Brad Yrhizobium Species

Carboxymethyl cellulase and polygalacturonase activities were evaluated in wild-type strains from different taxonomic groups of rhizobia which nodulate a specific range of legume hosts that do not normally overlap (R. meliloti, R. loti, R. leguminosarum biovar trifolii, R. leguminosarum biovar phaseoli, R. leguminosarum biovar viceae, and Bradyrhizobium japonicum). To detect these enzymatic act...

Genetic Structure of Rhizobium leguminosarum biovar trifolii and viciae Populations Found in Two Oregon Soils under Different Plant Communities.

An investigation was carried out to determine the genetic structure in soil populations of Rhizobium leguminosarum bv. trifolii and viciae at each of two Oregon sites (A and C) that were 1 km apart. Although the soils were similar, the plant communities were quite different because grazing by domestic animals had been allowed (site A) or prevented (site C). Analysis of allelic variation at 13 e...

trifolii . enzymes in Rhizobium leguminosarum biovar Cell - associated pectinolytic and cellulolytic

Typing of rhizobia by PCR DNA fingerprinting and PCR-restriction fragment length polymorphism analysis of chromosomal and symbiotic gene regions: application to Rhizobium leguminosarum and its different biovars.

Characterization of 43 strains of Rhizobium leguminosarum biovars viciae, trifolii, and phaseoli was performed by two methodologies based on PCR amplification, i.e., PCR DNA fingerprinting of interrepeat sequences and restriction fragment length polymorphism (RFLP) analysis of PCR -amplified chromosomal and symbiotic gene regions. Groupings generated by PCR DNA fingerprinting with either extrag...